Sample Preparation Using Solubilizers for Liquid Scintillation Counting

Preparation of whole blood for liquid scintillation counting.

Liquid scintillation counting of 3H-labeled whole-blood samples is severely impaired owing to quenching by blood pigments. In this study, dry oxidation and chemical solubilization followed by decolorization were the two general methods used to eliminate color quenching. Three blood volumes were examined: 0.25, 0.50, and 1.0 mL. Dry oxidation yielded complete recoveries of 3H label with counting...

Cocktails for Liquid Scintillation Counting

It is most important to assure 4 π−geometry in liquid scintillation counting for accurate results1. Usually this can be assured by dissolution of the sample in an appropriate cocktail. The cocktail does not only contain a solvent but also at least one scintillator and surfactants for aqueous samples because aqueous samples are completely insoluble in the pure organic solvents and scintillators....

Liquid Scintillation Counting

discussed, and he points out that merely because a disease is found to be familial in incidence does not mean it is hereditarily transmitted; leprosy, for instance, was long held to be a hereditary disease, and a law was actually proposed in the Norwegian Parliament forbidding the marriage of lepers for that reason. It was only with the discovery of the leprosy bacillus by Hansen that the true ...

Determination of accurate counting efficiency for liquid scintillation counting.

Rapid method for homogenizing tissue samples for liquid scintillation counting.

EVALUATiON OF A new drug includes analysis of its tissue distribution. We have developed an improved method for solubilizing tissues that is not only rapid, but results in a homogeneous sample for counting. We had found that organs rich in connective tissue (heart, lung, skeletal muscle, urinary bladder, stomach, and intestinal wall) could not be completely disrupted by conventional techniques ...